Abstract: Taking ‘Longcheng No.2’ soft jujube kiwifruit as the test material, four treatments of control（CK）,nitrogen（-N）,phosphorus（-P） and potassium（-K） deficiencies were set up in hydroponics to measure the growth and physiological characteristics of soft jujube seedlings, effects of macronutrient deficiencies on the growth and gas-exchange parameters of soft jujube kiwifruit seedlings were studied, in order to provide reference for diagnosis of deficiencies in soft jujube kiwifruit and for the scientific management of the soft jujube kiwifruit.The results showed that the plant height, basal diameter and root volume of jujube seedlings were inhibited by N,P and K deficiency.It also resulted in the net photosynthetic rate（Pn）,stomatal conductance（Gs）,transpiration rate（Tr） and intercellular carbon dioxide concentration of kiwifruit seedlings.Ci, maximum photochemical quantum efficiency（Fv/Fm）,potential photochemical efficiency（Fv/Fo）,photochemical quenching coefficient（qP）,actual photochemical efficiency（ΦPSⅡ） and electron transport rate（ETR） were significantly lower than those of CK,but non-photochemical quenching coefficient（qN） was significantly higher than that of CK（P＜0.05）.Therefore, the lack of a large number of elements led to the inhibition of non-stomatal photosynthesis in kiwifruit, and the reduction of carbon dioxide assimilation efficiency, which significantly affected photosynthesis, respiration and gas exchange, and inhibited its normal growth.