“贵长”猕猴桃蒂腐病病原菌多样性及致病力测定

雷霁卿; 曾傲敏; 刘颖; 曹森; 王瑞

“贵长”猕猴桃蒂腐病病原菌多样性及致病力测定

Determination of Stem-end Rot Pathogen Diversity and Pathogenicity of ‘Guichang’ Kiwifruit

摘要

摘要: 以“贵长”猕猴桃病果为试材，通过病原菌分离、形态学鉴定、分子生物学鉴定及致病力测定，研究了“贵长”猕猴桃蒂腐病病原菌多样性及致病力差异，以期为猕猴桃蒂腐病防控提供参考依据。结果表明：“贵长”猕猴桃蒂腐病病原菌有4类，8株，分别是小新壳梭胞菌属(Neofusicoccum sp.)、间座壳菌属(Diaporthe sp.)、葡萄座腔菌(Botryosphaeria sp.)、盘多毛胞菌属(Pestalotiopsis sp.),各菌属检出率为25.0%、50.0%、12.5%、12.5%;将菌株序列上传至GenBank,获得登录号MK5875050-MK587753、MK587756、MK587760、MK587762、MK5875063;聚类分析将8株病原菌致病力分为强致病力(37.5%)、中致病力(25.0%)和弱致病力(37.5%),各菌株致病力差异与其所属菌属无相关性。

Abstract: Taking ‘Guichang’ kiwifruit as the test material, the stem-end rot pathogens′ diversity and pathogenicity of ‘Guichang’ kiwifruit were studied by pathogen isolation, morphological identification, molecular biological identification and pathogenicity determination, in order to provide reference for the prevention of the kiwifruit stem-end rot.The results showed that there were 8 pathogen strains belonging to 4 categories, respectively are Neofusicoccum sp.,Diaporthe sp.,Botryosphaeria sp.and Pestalotiopsis sp.,and the detection rates were 25.0%,50.0%,12.5%,12.5%.The strain sequence was uploaded to GeneBank and login numbers were MK5875050-MK587753,MK587756,MK587760,MK587762,MK5875063.The pathogenicity of 8 isolates was classified into strong pathogenicity（37.5%）,moderate pathogenicity（25.0%） and weak pathogenicity（37.5%） by cluster analysis.There was no correlation between the pathogenicity of each strain and its genus.

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