Abstract: Taking Qinghai wild Hippophae rhamnoides subsp.sinensis Rousi rhizosphere soil as material, strains with certain growth promoting ability were screened out.The rhizosphere growth promoting bacteria of wild Hippophae rhamnoides subsp.sinensis Rousi were isolated by traditional culture method, and the strains were purified by plate scribing method, in order to create conditions for excavating high-efficiency rhizosphere growth promoting strains with potential application value.The results showed that morphological characteristics, physiological and biochemical tests, and 16S rDNA,the phosphorus removal capacity of the isolated strains was determined by the phosphorus removal method, and the potassium removal capacity was determined by the atomic absorption meter.Four strains of bacteria were isolated.According to the physiological, biochemical and 16S rDNA molecular identification results, the four strains belonged to Pseudomonas.The ability test results showed that the radius of K7 dissolved organic phosphorus was 13.71 mm, the radius of K28 dissolved inorganic phosphorus was 7.62 mm, and the potassium dissolution rate of K39 was 14.33 μg·mL^-1.The three strains obtained were efficient strains for dissolving organic phosphorus, inorganic phosphorus and potassium respectively, indicating that the three strains could be used as potential materials for developing high-efficiency bacterial fertilizer.