Abstract: Taking ‘Ningqi No.7’ as the experimental material, the effects of Trichoderma biofertilizer（A1,A2,A3,A4,A5,CK） with different dosage on the morphological characteristics of Lycium barbarum,physiological indexes of Lycium barbarum,fruit quality and yield, and physical and chemical properties of Lycium barbarum rhizosphere soil were studied, in order to provide reference for improving the yield and quality of Lycium barbarum in organic Lycium barbarum orchard.The results showed that, 1) compared with CK,the photosynthetic characteristics of leaves of Lycium barbarum were improved after application of Trichoderma biofertilizer, and the plant height, crown width, ground diameter and fruity branch length of Lycium barbarum were significantly increased, and A3 and A4 treatments had the best improvement effect.2) After the application of Trichoderma biofertilizer, the yield of each fertilizer treatment was significantly increased by 11.9%-35.7% compared with CK,and the fruit quality of Lycium barbarum was improved.The contents of total sugar, Lycium barbarum polysaccharide, betaine, total flavonoids, carotenoid, ascorbic acid and polyphenol in Lycium barbarum fruits were significantly increased compared with CK,respectively, and A3 treatment had the best effect.3) The application of Trichoderma biofertilizer reduced the soil conductivity and increased the contents of soil organic matter, total nitrogen, total phosphorus, total potassium, alkali-hydrolyzed nitrogen, available phosphorus and available potassium, and the A3 treatment had the most obvious soil improvement effect.After comprehensive evaluation, it was concluded that the growth conditions of Lycium barbarum and soil physicochemical properties were improved under different dosages of Trichoderma biofertilizer.Among them, the A3(44 550 mL·hm^-2) biofertilizer could significantly affect the growth and soil properties of Lycium barbarum and would not be wasted.