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叶面喷施钙素对NaHCO3胁迫下油菜幼苗生长及生理特性的影响

Effects of Foliar Spraying Calcium on Growth and Physiological Characteristics of Rape Seedlings Under Alkali Stress

  • 摘要: 以“华油杂62(耐盐型)”与“湘油15(敏盐型)”为试材,采用盆栽试验,研究了100 mmol·L-1 NaHCO3胁迫下,油菜幼苗叶面喷施不同浓度CaCl2对其生长及生理特性的影响,以期为油菜在盐碱地的栽培提供参考依据。结果表明:叶面喷施1%和0.5%CaCl2“华油杂62”与“湘油15”茎叶鲜质量,较NaHCO3胁迫分别显著增加了117.58%与88.89%。叶面喷施2%CaCl2显著(P<0.05)提高了“华油杂62”叶片潜在光化学效率(Fv/Fo),较NaHCO3胁迫提高了35.58%,而“湘油15”在钙浓度为1%时,Fv/Fo的提高最为明显,较NaHCO3胁迫提高了19.60%。叶面喷施0.5%CaCl2显著(P<0.05)提高了“湘油15”茎叶组织中K+/Na+,较NaHCO3胁迫增加了58.76%;“华油杂62”叶面喷施1%CaCl2,K+/Na+增加最为明显,较NaHCO3胁迫增加了7.12%。叶面喷施0.5%CaCl2,显著提高了“湘油15”的叶片超氧化物歧化酶(SOD)活性,较NaHCO3胁迫提高了66.21%,“华油杂62”叶面喷施1%CaCl2,SOD活性较NaHCO3胁迫提高了19.36%。综上,叶面喷施适宜浓度CaCl2可以显著促进NaHCO3胁迫下不同耐盐性油菜幼苗的生长,提高其抗逆性,适宜的CaCl2浓度范围为0.5%~1%。

     

    Abstract: A pot experiment was conducted with ‘Huayouza 62(salt-tolerant)’ and ‘Xiangyou 15(salt-sensitive)’ as the test material.The effects of folior spraying CaCl2 with different concentrations on the growth and physiological characteristics of rape seedlings under 100 mmol·L-1 NaHCO3 stress were investigated, in order to provide reference for rape cultivation in saline-alkali land.The results showed that when the calcium concentration was 1.0% and 0.5%,the corresponding fresh weight of stems and leaves of ‘Huayouza 62’ and ‘Xiangyou 15’ were increased significantly(P<0.05),which were 117.58% and 88.89% compared with NaHCO3 stress.When the calcium concentration was 2.0%,the potential photochemical efficiency(Fv/Fo) of leaves of ‘Huayouza 62’ was significantly increased(P<0.05),which was increased by 35.58% compared with NaHCO3 stress.When the calcium concentration was 1.0%,the Fv/Fo of ‘Xiangyou 15’ was increased most obviously, which was increased by 19.60% compared with NaHCO3 stress.When calcium concentration was 0.5%,K+/Na+ in stems and leaves tissues of ‘Xiangyou 15’ was significantly increased(P<0.05),which was increased by 58.76% compared with NaHCO3 stress.When calcium concentration was 1.0%,K+/Na+ of ‘Huayouza 62’ was significantly increased(P<0.05),which was increased by 7.12% compared with NaHCO3 stress.When the calcium concentration was 0.5%,the SOD activity in leaves of ‘Xiangyou 15’ was significantly increased(P<0.05),which was increased by 66.21% compared with NaHCO3 stress.When the calcium concentration was 1.0%,the SOD activity in leaves of ‘Huayouza 62’ was significantly increased(P<0.05),which was increased by 19.36% compared with NaHCO3 stress.In conclusion, the suitable concentration of CaCl2 was sprayed on the leaves, it could significantly promote the growth of different salt-tolerant rape seedlings under NaHCO3 stress, and improve their stress resistance.The appropriate CaCl2 concentration range was 0.5%-1.0%.

     

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