Abstract: Taking symptomatic fruits of a serious muskmelon Fusarium wilt（FWM） in fields as material, the pathogen was isolated by tissue separation, and identified via morphological and molecular methods.The pathogenicity of the identified pathogen was verified by Koch′s rule.In addition, the antifungal activity of eight kinds of chemical agents commonly used against the pthogen in production were detected by mycelium growth rate method indoor, in order to provide help for FWM control in field using highly effective and low-toxic chemical pesticides.The results showed that Fusarium verticillioides was identified as a novel causing agent of FWM in Jilin Province, and the prochloraz gave highest inhibitory activities against F.verticillioides among eight tested chemical agents, which EC₅₀ was 0.029 5 μg·mL^-1,and the which of the tebuconazole and flusilazole were 0.146 7 μg·mL^-1 and 0.190 1 μg·mL^-1,respectively.The minimum inhibitory concentration of prochloraz, tebuconazole and flusilazole were 0.048 μg·mL^-1,1.2 μg·mL^-1 and 0.8 μg·mL^-1,respectively.The mininal bactericidal concentration of prochloraz, tebuconazole and flusilazole were 0.052 μg·mL^-1,1.6 μg·mL^-1 and 0.8 μg·mL^-1,respectively.The results of indoor control effects indicated that at the concentration of 0.144 μg·mL^-1,prochloraz indicated the highest control efficiency of 82.88% against FWM.