Abstract: The leaf buds of apple variety ‘2001’ were used as test materials, the soluble protein, soluble sugar, starch, ABA,IAA,ZT,GA₃,SOD,POD,CAT were tested to study nutrients, endogenous hormone content and enzyme activities during the initial germination of apple leaf buds.So as to regulate apple leaf buds germination and guide pruning.The results showed that during the germination of apple leaf buds, the starch content showed a slow decline process, the soluble sugar content showed a rise process, and the soluble protein content showed a process of the first decline and then rise.It reached 4.34,49.17,52.75 mg·g^-1;the content of endogenous hormone IAA showed an increasing trend overall, the content of ABA was the opposite, and gradually decreased during leaf buds germination, and the contents of ZT and GA₃ increased first and then lowering process.The IAA content reached the highest value of 101.70 ng·g^-1FW on the last day within 20 days of observation, and the GA₃ content reached the highest value of 22.35 ng·g^-1FW on the 8^th day.During leaf buds germination, SOD activity reached 35.12 U·g^-1,the activities of POD and CAT reached 11.40,0.25 U·g^-1·min^-1 on the 20^th day, respectively.