Abstract: Cymbidium with variegated leaf（Cymbidium tracyanum var.‘huanghua’ × C.iridioides D.Don.‘Yeyi’） was used as the experimental material, based on the EST sequence of up-regulated expression of CcMYB24 gene obtained by our research group.The full length of CcMYB24 gene was amplified by PCR,and the function of CcMYB24 gene was studied by bioinformatics analysis and subcellular localization, in order to provide reference for the utillization of the structure and fundion of plant MYB24.The results showed that CcMYB24 gene was 908 bp and encoded 301 amino acids.Bioinformatics analysis predicted that CcMYB24 protein was alkaline, unstable, hydrophilic and non-transmembrane non-secretory protein.The CcMYB24 amino acid sequence had a specific matched conserved MYB protein DNA binding domain and contains two R structures, which belonged to the R2R3-MYBs family.The results of subcellular localization showed that CcMYB24 protein was located on the nucleus.Phylogenetic tree showed that it was closely related to Cymbidium ensifolium and had the highest homology with CeRAX2-like of Cymbidium ensifolium（95.99%）.Since CcMYB24 belonged to the R2R3-MYBs family, it was speculated that CcMYB24 transcription factor might play a similar role to R2R3-MYB transcription factor.