Abstract: Twenty excellent kiwifruit varieties were planted in Guiyang area.The indexes of phenological period, fruiting habit, fruit appearance and intrinsic quality of different varieties were comprehensively evaluated, their performance was observed, in order to select kiwifruit varieties suitable for the climate and comprehensive traits in the middle of Guizhou for promotion and application in production.The results showed that 20 varieties could complete the entire growth cycle in the test area, and could produce fruit normally, with small inter-annual differences.The flowering period of ‘Jinyi’ were the earliest, and the ripening time of the Actinidia arguta ‘Ruby Star’ and ‘Kuilyu’ was the earliest, and the ripening time of the remaining varieties was after mid to late September.The tested varieties showed good fruiting habits, but there was a certain difference in resistance to kiwifruit canker disease, of which ‘Ziyu’ ‘Chuhong’ ‘Cuiyu’,and ‘Jinkui’ showed good high-yield performance, 4 copies the Actinidia arguta kiwifruit canker disease resistance were high resistance, ‘Jinkui’‘Xuxiang’‘Cuiyu’‘Kuimi’‘Jinyang’ and ‘Yulinglong’ were more resistant to kiwifruit canker disease.There were differences in the appearance and intrinsic quality performance of the tested varieties.The soluble solid contents of ‘Jinkui’‘Xuxiang’‘Kuilyu’‘Jinyi’‘Cuiyu’ and ‘Chuhong’ were as high as above 16.5%,the flavor was sweet and sour, and the aroma was strong.The contents of soluble sugar and vitamin C in ‘Yulinglong’ were the highest, and the flesh was delicate.Comprehensive evaluation of resistance to kiwifruit canker disease, fruit quality of kiwifruit varieties and consideration of indicators such as fruiting characteristics, Actinidia chinensis Planch ‘Jinyi’（early ripening, yellow meat）,‘Chuhong’（early ripening, red meat） and ‘Cuiyu’（medium ripening, green meat）, Actinidia chinensis var.‘Xuxiang’（medium ripeing, green meat） and ‘Jinkui’ （middle-late cooked,green meat）were suitable for popularization in the middle of Guizhou;Actinidia arguta kiwifruit‘Kuilyu’（very early ripening,green flesh）,‘Ruby Star’（very early ripening,red flesh）and‘Ziyu’（very early ripening,red flesh）and Actinidia eriantha Benth.‘Yulinglong’（late ripening,green meat）suitable as a characteristic variety.