Abstract: With the optimization and upgrading of industrial structure, the subjects in the industry gradually change from the state of competition and disorder imbalance to the trend of mutual benefit and value co-creation.The Likert scale and SEM model were used to study the influence path of innovation subjects in the innovation ecosystem of peach industry in Shunping county on the collaborative evolution and development of the innovation ecosystem of peach industry, which was helpful to promote the high-quality development of peach industry and realize the collaborative symbiosis between subjects.The results showed that the variety innovation ability of innovation subjects, the support of service organizations and the sales and circulation ability of fresh peach trading market had a significant positive correlation with the co-evolution development of innovation ecosystem of peach industry.Technological innovation capability and consumer demand level had no positive correlation with the co-evolution development of innovation ecosystem of peach industry, and had no direct and significant impact on the co-evolution development of the system.According to the research results, this study put forward countermeasures and suggestions on improving innovation ability, perfecting service support, improving the level of market sales and circulation, and taking consumer demand as the guidance.