Abstract: The Guangxi local variety Andrographis paniculata was used as test material,and the greenhouse potting method was adopted,five commonly used plant growth regulators in production were selected to research the effects of different plant growth regulators on morphological characters and dry matter accumulation of Androgrcaphis paniculata,including NAA,6-BA,eBR,CCC and GA₃,in order to provide reference for increasing the yield of Chinese medicinal materials.The results showed that the treatments of 200 mg·L^-1,400 mg·L^-1 and 600 mg·L^-1 GA₃ could significantly increase the plant height of Andrographis paniculata（P＜0.05）,and the leaves were obviously enlarged.Among them,spraying 400 mg·L^-1 GA₃ increased the plant height extremely significantly（P＜0.01）,and spraying 400 mg·L-1 GA3,the biomass of single plant significantly increased by20.97%（P＜0.05）,but the leaf-to-stem ratio decreased while increasing the biomass per plant.Although the biomass of single plant of Andrographis paniculatain each CCC treatments decreased in different degrees,50 mg·L-1 CCC could significantly increase the leaf-to-stem ratio（P＜0.05）.Each treatment concentration of 6-BA and NAA inhibited dry matter accumulation on Andrographis paniculata.In summary,spraying 400 mg·L-1 GA3 can significantly increase the biomass of single plant,but 6-BA and NAA are not suitable for the production of Andrographispaniculata.