Abstract: The branches of ‘Hongyang’ kiwifruit in Dali prefecture were used as materials.We used the method of real investigation to survey the damage status,isolated the pathogenes of 10 samples gathering by adopting usual tissue isolation,purified single spore to gain pathogenic bacteria,morphological and molecular identification were used to identify pathogen types.The experiment was carried out to investigate the harmfulness and pathogen causing.The results showed that,stem diseases of‘Hongyang’kiwifruit in Dali prefecture affected seriously,the incidence of disease was69.6%.There were 15 bacterial strains isolated from 5 sampling points.Four kinds of dominant bacteria had higher frequency of separation rate,they were Alternaria alternata（55.6%）,Fusarium chlamydosporum（52.4%）,Neofusicoccum parvum（49.8%）and one of Diaporthe bohemiae（50.1%）.And Koch′s Rule proved the stem diseases of kiwifruit were caused by the above dominant strains,further clarify the pathogenic fungi that caused stems of Dali prefecture kiwifruit.