Abstract: The culture medium of Antrodia cinnamomea mycelium was prepared with the extracts of five kinds of Juncao, including Cymbopogon citratus,Clinacanthus nutans,Pennisetum giganteum,Pennisetum purpureum and Pennisetum purpureum Schumab cv.Purple.The mycelium growth rate, triterpene, amino acids, extracellular enzyme activity and the key genes expression of triterpene synthesis pathway were used to screen out the culture medium promoting the mycelium growth and active substances accumulation of Antrodia cinnamomea,in order to provide reference to solve the problems of high cost and long time-consuming of artificial culture of Antrodia cinnamomea in the future.The extracts of Cymbopogon citratus and Clinacanthus nutans were significantly promoted the growth of mycelium of Antrodia camphorata,and the cultivation time was 12 days shorter than the control.The promotion effect of different mycorrhizal extracts on the triterpene content of Antrodia camphorata were as follows, XMC＞JJC＞YDC＞ZSXC＞XC,which were significantly different with the control; Cymbopogon citratus significantly promoted the synthesis of laccase and glutamate synthase, and significantly promoted the expression of AcHMGS,AcHMGR and AcSE.Cymbopogon citratus could be also used as mycelia culture medium of Antrodia camphorata to promote the growth and the accumulation of triterpene content.