Abstract: The budding stems of Impatiens rubrostriata were used as materials.The explants were treated with different sterilants,and primary culture,proliferation and rooting culture.They were performed on the medium with different types of hormoneand concentrations to screen the best sterilization method,induction,proliferation and rooting medium of Impatiens rubrostriata.The results showed that the optimal sterilization method for Impatiens rubrostriatais 75% alcohol,10 seconds+0.1% mercury chloride for 6 minutes,the survival rate was 100%.When 20 mg·L^-1cefotaxime sodium was added to the primary medium,the bacterial effect was better,the survival rate was 100%.The most suitable primary medium was MS+1.0 mg·L^-1 6-BA+0.3 mg·L^-1 NAA,under this circumstances,the seedlings were strong and the growth was fast.The optimal proliferation medium was MS+1.0 mg·L^-1 6-BA+0.3 mg·L^-1NAA+0.010 mg·L^-1TDZ,the proliferation coefficient was 6.65,the seedlings grew fast.The most suitable rooting medium was MS+0.10 mg·L^-1 IBA,and the rooting rate was 100%.The in vitro rapid propagation system for Impatiens rubrostriata was established,which will lay a good technical foundation for development and utilization of Impatiens rubrostriata.