花毛茛组培快繁技术研究

Study on Rapid Technique of Tissue Culture of Ranunculus asiaticus

摘要: 以花毛茛初代培养无菌苗为试材,采用MS培养基,分别向培养基中添加不同配比的细胞分裂素6-BA和细胞生长素NAA,对花毛茛进行初代诱导、增殖培养,继代生根培养,并以不同比例泥炭、蛭石、珍珠岩、河沙为基质,对花毛茛进行炼苗移栽试验。结果表明:最佳初代诱芽培养基为MS+1.5 mg·L^-1 6-BA+0.2 mg·L^-1 NAA,诱导率达100%;增殖诱导愈伤组织培养基为MS+0.5 mg·L^-1 6-BA+0.1 mg·L^-1 NAA,增殖系数为5.0;继代生根培养基为1/2MS+0.2 mg·L^-1 NAA,生根率89.9%;移栽最适基质为泥炭∶河沙∶蛭石=6∶2∶2,成活率100%。

Abstract: The sterile seedlings of Ranunculus asiaticus were cultured on MS medium supplemented with 6-BA and NAA respectively to induce and proliferate in the primary generation of Ranunculus asiaticus,the experiment of Ranunculus asiaticus seedlings transplanting was carried out by subculture of rooting and different proportion of peat,vermiculite,perlite and river sand.The results showed that the best medium for bud induction was MS+1.5 mg·L^-1 6-BA+0.2 mg·L^-1 NAA,the induction rate was 100%,the medium for callus induction was MS+0.5 mg· L-1 6-BA +0.1 mg·L-1 NAA,the multiplication coefficient was 5.0.The subculture medium for rooting was1/2 MS+0.2 mg·L-1 NAA,the rooting rate was 89.9%,the best medium for transplanting was peat+river sand+vermiculite=6∶2∶2,survival rate was 100%.