Abstract: Taking Chrysanthemum morifolium leaves as the test material, the rapid propagation system of Chrysanthemum morifolium was stuided by orthogonal design method.After subculture, the plant height and ground stem were determined as quantitative indicators for the final classfication.The results showed that the best sterilization plan was 0.1% mercury liter treatment for 3 minutes, in order to provide a reference for the standardized planting of Chrysanthemum morifolium and the formulation of general standards for seedlings.The best medium for adventitious bud induction was MS+2.0 mg·L^-1 6-BA+0.1 mg·L^-1 NAA+20 g·L^-1 sucrose, its induction rate reached 88%.The best rooting medium was 1/2 MS+0.1 mg·L^-1 IBA,and its rooting rate was 100%.Through cluster analysis, Chrysanthemum morifolium tissue cultured seedlings were divided into Level Ⅰ,Level Ⅱ and Level Ⅲ,the grade standard for Grade I tissue culture seedlings were plant height≥5 cm, ground stem≥1.16 mm, a total of 23 plants.Grade Ⅱ tissue culture seedlings were 4 cm≤plant height＜5 cm, 1 mm≤ground stem＜1.16 mm, a total of 40 plants.Level Ⅲ tissue culture seedlings were 1 cm≤plant height＜4 cm, ground stem＜1 mm, a total of 17 plants, in line with the normal distribution.