Abstract: Epimedium sagittatum were used as experimental materials, the effect of Bacillus licheniformis（A）,humic acid（B）,fulvic acid potassium（C） and compound fertilizer（D） on the quality of E.sagittatum was investigated by using L₉（3~4） orthogonal experimental design and principal component analysis, and the effect of different types and amounts of base fertilizer on the content of E.sagittatum components was investigated by using Minitab 19.The results were analyzed using Minitab 19 to obtain the response of each index component to each factor level of the fertilizer and the influence of each factor level on each index component, in order to screen the best fertilizer ratio and provide reference for the construction of a standardized planting base of high-quality E.sagittatum.The results showed that compared with the control, the content of total flavonoids in treatment group 5 of E.sagittatum was the highest when the four factors were combined, which increased by 7.91%.The content of epimedin A and epimedin B in treatment group 4 increased by 21.43% and 23.53% respectively.In treatment group 6,the contents of epimedin C,Icariin and total flavonol glycosides increased by 12.15%,14.80% and 12.20% respectively.The total flavone content was significantly increased by the combination of A₂B₂C₃D₂ factor levels; The content of epimedin A and epimedin B could be significantly increased by the combined application of A₂B₁C₂D₁ factor level; Combined application of A₂B₃C₁D₂ factor level could significantly increase the content of epimedin C and total flavonol glycosides.According to the results of orthogonal test, Taguchi analysis and principal component analysis, the best fertilizer combination to improve the quality of E.sagittatum was treatment group 6 A₂B₃C₁D_2,Bacillus licheniformis 270 mL·（667m~2）^-1+humic acid 10 L·（667m~2）^-1+fulvic acid potassium 800 g·（667m~2）^-1+compound fertilizer 7.5 kg·（667m~2）^-1.Treatment group 6 A₂B₃C₁D₂ could be used as the base fertilizer fertilization scheme for the growth stage of E.sagittatum.