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嫁接茄子不同器官丙酮浸提液对黄萎病诱导抗性的影响

Effects of Acetone Extracts From Different Organs of Grafted Eggplant on Induced Resistance to Verticillium wilt of Eggplant

  • 摘要: 以嫁接茄子植株不同器官(根、茎、叶)为试材,用丙酮分别对其进行浸提,采用自然病原激发病害试验与人工接种病原激发病害试验相结合的方法,研究了茄子幼苗期(2叶1心)在其诱导处理(灌根)后对植株病株率、病情指数以及诱导抗性效果的影响,以期为农业生产中对茄子黄萎病进行绿色防控提供参考依据。结果表明:在自然病原激发病害试验中,经过丙根、丙茎与丙叶诱导处理后茄子植株病株率与丙CK相比分别降低31.82%~100.00%、18.21%~33.74%、9.90%~66.93%,病情指数分别降低34.40%~100.00%、18.98%~52.65%、10.03%~56.86%,平均诱导抗性效果达到76.33%、64.88%、58.44%,与丙CK均有显著或极显著差异;在人工接种病原菌激发病害试验中,经过丙根、丙茎与丙叶诱导处理后茄子植株病株率与丙CK相比分别降低16.89%~41.99%、13.40%~25.57%、8.06%~19.20%,病情指数分别降低19.00%~46.23%、11.36%~32.59%、6.52%~42.97%,平均诱导抗性效果达到68.70%、63.32%、62.48%,与丙CK均有显著或极显著差异。自然病原激发病害试验与人工接种病原菌激发病害试验中,诱导抗性效果强弱趋势均为丙根处理>丙茎处理>丙叶处理。

     

    Abstract: Different organs(roots, stems and leaves) of grafted eggplant plants were used as test materials, and were extracted with acetone respectively.The combination of natural pathogen-induced disease test and artificial inoculation pathogen-induced disease test.The effects of induction treatment(irrigated roots) on the diseased plant rate, disease index and induction resistance of eggplant at the seedling stage(2 leaves and 1 heart) were studied, in order to provide reference for green prevention and control of eggplant Verticillium wilt in agricultural production.The results showed that in the experiment of natural pathogen exciting diseases, compared with the control, the disease rate of eggplant plants induced by acetone extracts of grafted eggplant roots, grafted eggplant stems and grafted eggplant leaves decreased by 31.82%-100.00%,18.21%-33.74% and 9.90%-66.93%,respectively.Disease index decreased by 34.40%-100.00%,18.98%-52.65%,10.03%-56.86%.The average induced resistance effect reached 76.33%,64.88% and 58.44%,which were significantly or extremely significantly different from the acetone CK;in the experiment of artificial inoculation of pathogenic bacteria to stimulate diseases, compared with the acetone CK,the disease rate of eggplant plants induced by acetone extracts of grafted eggplant roots, stems and leaves decreased by 16.89%-41.99%,13.40%-25.57% and 8.06%-19.20%,respectively, the disease index decreased by 19.00%-46.23%,11.36%-32.59% and 6.52%-42.97% respectively, the average induced resistance effect reached 68.70%,63.32% and 62.48%,which were significantly or extremely significantly different from the acetone CK.In the natural pathogen excitation disease test and artificial inoculation pathogen excitation disease test, the trend of induced resistance was acetone extract of grafted eggplant roots>acetone extract of grafted eggplant stems>acetone extract of grafted eggplant leaves.

     

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