Abstract: Taking degraded soil and normal soil from Shuangyashan City, Heilongjiang Province as test materials, the community structure and functional differences of 16S rDNA V4-V5 bacterial communities in degraded soil and normal soil samples were studied using Illumina high-throughput sequencing method, in order to investigate the reasons why Handkea utriformis can not grow in degraded soil and provide reference for artificial domestication of Handkea utriformis.The results showed that the operational taxonomic units belong to 2 kingdoms, 44 phyla, 128 classes, 299 orders, 508 families, 1 048 genera.Richness index（Chao1 and ACE） and diversity index（Shannon and InvSimpson） of degenerated soil bacterial communities all decreased.In the degenerated soil, the relative abundances of Bacteroidetes, Gemmatimonadetes, Planctomycetes, Pseudarthrobacter,RB41,Terrimonas decreased, and the relative abundances of Verrucomicrobia, Acidothermus,Rhodanobacter increased.LEfSe analysis results showed that in CK soil, 12 dominant bacterias were Alphaproteobacteria, AD3,Acidobacteria, Ktedonobacterales, Rhizobiales, Gaiellales, Micropepsales, Micropepsaceae, Ktedonobacteraceae, Rhodanobacteraceae, HSB OF53-F07,Candidatus Solibacter.2 metabolic pathways of the primary functional layers were the metabolism and organic system, 6 sub function layers were biosynthesis of other secondary metabolites, energy metabolism, metabolism of cofactors and vitamins, metabolism of terpenoids and polyketones, circulatory system and digestive system.Compared with normal soil, 3 primary metabolic pathways and 8 secondary metabolic pathways were reduced.The structural changes of bacterial community structure in the degenerated soil and the accompanying changes in metabolic function were the important reasons for the inability of the Handkea utriformis continue to grow.