Abstract: Taking 70 chrysanthemum germplasm as experimental materials, 19 agronomic characters were comprehensive evaluation and screening of elite germplasm accessions by diversity index, subordinative function value, correlation analysis, principal component analysis, cluster analysis, which would provide some parental materials and reference for breeding of new chrysanthemum germplasm.The results showed that 19 phenotypic traits revealed a higher genetic diversity in this collection.The genetic diversity indexes were from 1.28 to 2.20,and more than 2.00 were respectively first branch height, branch number per plant, layer of ray floret, ray floret number, tubiform floret diameter, tubiform floret number, they could be considered as an evaluation index for breeding of new cultivars and genetic improvement.The variable coefficient was from 23.23%-83.63%,in which the variation coefficient of flower number per plant were the largest of 83.63%,and the variation coefficient of leaf length was the smallest of 23.23%.Flower related traits existed different degrees of correlation, most of which were significant or highly significant correlative.Principal component analysis showed the accumulative contribution rate of the first 7 principlal component were 24.67%,15.43%,12.63%,7.20%,6.19%,5.75%,5.43%,respectively; which represented 77.31% information of 19 phenotypic characters.Cluster analysis showed that the 70 medicinal chrysanthemum germplasm were divided into four groups when the genetic distance was 10.0.Group 1 represented bigger tubular flower diameter, shorter ligulate flower, medium inflorescences diameter and yellow flower.Group 2 represented low tubular flower diameter, shorter ligulate flower, small white flower.Group 3 represented low tubular flower diameter, longer ligulate flower, large leaves, large yellow flower.Group 4 represented bigger tubular flower diameter, shorter ligulate flower, bigger plant, many white or pinkish purple small flower.Selection of ‘Huai Chrysanthemum 14’‘Tongxu Honggan Low Yellow Chrysanthemum’‘Henan Huai Chrysanthemum’‘Jinsihuang Chrysanthemum’ were applied to breeding of new cultivars and genetic improvement for chrysanthemum.