Abstract: To determine the effects of different casing soils on the fruiting and yield of Oudemansiella raphanipes,loam, sand and peat commonly used in production were crossing mixed as casing soil to cultivate O.raphanipes,and the fruiting time, yield, agronomic traits and nutrients of fruiting bodies were evaluated.Results showed that the fruiting bodies could normally produced treated with the four kinds of casing soils.When peat was added into the casing soil, the primordia and fruiting bodies appeared earlier than that without peat, and the mushroom production period was shortened.Specifically, 50% peat mixed with 50% loam was the most suitable casing soil in this study for the growth and development of the fruiting body of O.raphanipes,resulting in the earliest primordia, the shortest production period, the best agronomic traits, and the highest yield and biotransformation rate with 275.08 g and 50.01% per artifical bed-log on average, which could be applied to practical production.The results here will provide a theoretical basis for the scientific cultivation of O.raphanipes.