Abstract: Taking Chinese cabbage as the test material, the seedlings were raised in cavity trays.The effects of different substrate ratios on various physiological indicators（plant height, stem thickness, root length, total leaf area, seedling strength index and root to crown ratio） of Chinese cabbage were investigated with sludge, mushroom residue and Chinese medicinal residue as raw materials and commercially available commercial substrates（grass charcoal∶vermiculite∶perlite=3∶1∶1） as control, in order to provide reference for the selection of alternative substrates for Chinese cabbage seedlings.The results showed that the substrate with the ratio of sludge∶mushroom residue∶Chinese medicinal residue=70∶30∶5 contained 0.13 g·kg^-1,568.23 mg·kg^-1 and 119.38 mg·kg^-1 of effective nitrogen, effective phosphorus and effective potassium, which had the best effect on the growth of Chinese cabbage.It produced 2.05,2.43,1.15,2.48,1.42 times and 1.47 times stronger seedling index, biomass, root to crown ratio, leaf area, stem thickness and plant height of Chinese cabbage than the control, respectively.It could partially replace the grass charcoal substrate as seedling substrate and reduce the cost of cabbage seedlings.