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菜豆立枯病室内种质抗性评价与杀菌剂筛选

Indoor Germplasm Resistance Evaluation and Fungicide Screening of Common Bean (Phaseolus vulgaris L.) Blight

  • 摘要: 以田间采集的发病植株和48份菜豆种质资源为试材,采用分子生物学和形态学相结合,室内人工接种病原菌筛选菜豆抗性资源以及杀菌剂毒力测定的方法,研究了致病病原菌立枯丝核菌对菜豆生长的影响,以期筛选黑龙江地区菜豆立枯病的抗性资源以及有效防治方法。结果表明:该病原菌株为立枯丝核菌(Rhizoctonia solani);在48份菜豆种质资源的抗性筛选中发现2份中抗种质资源,分别为“泰优18号玉豆”和“早无筋架豆”;14种杀菌剂对该菌株均有抑制作用,其中肟菌·戊唑醇(0.03 mg·L-1)、腐霉利(0.4 mg·L-1)、醚菌·啶酰菌(10.6 mg·L-1)和戊唑·嘧菌酯(4.8 mg·L-1)的EC50值较小,抑菌效果较好。

     

    Abstract: The pathogenic plants collected in the field and 48 germplasm resources of common bean were used as test materials, and the effects of pathogenic pathogen Rhizoctonia solani on the growth of common bean were studied by the combination of molecular biology and morphology, indoor artificial inoculation of pathogenic bacteria to screen resistance resources and the toxicity of fungicides, in order to screen resistance resources and effective control methods of common bean blight in Heilongjiang region.The results showed that the pathogen was Rhizoctonia solani.In the resistance screening of 48 common bean germplasm resources, two moderately resistant germplasm resources were found, namely ‘Taiyou No.18 Jade Bean’ and ‘Zao Wujin Jie bean’.All of the 14 fungicides had inhibitory effects on the strain, among which the EC50 values of trifloxystrobin tebuconazole(0.03 mg·L-1),procymidone(0.4 mg·L-1),kresoxim-methyl boscalid(10.6 mg·L-1) and tebuconazole azoxystrobin(4.8 mg·L-1) were relatively small, and the antibacterial effect was better.

     

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