Abstract: Taking Cannabis sativa L.as the test material, the seedlings were treated with salt solution of different concentrations(100,200,300,400 mmol·L^-1),normal growing seedlings were used as control（CK）,the changes of physiological indexes, and contents of secondary metabolites such as total flavonoids and cannabidiol were observed.The physiological and biochemical characteristics of C.sativa under salt（NaCl） stress and the effects of secondary metabolites content were studied, in order to provide reference for cultivation and salt-tolerance adaptability of C.sativa.The results showed that the net photosynthetic rate, stomatal conductance, transpiration rate, maximum photochemical efficiency（Fv/Fm） and photosynthetic pigment decreased continuously with the increase of salt concentration and the prolongation of stress time.The contents of malondialdehyde and proline increased gradually with the increase of salt concentration（except proline under N4 treatment）.The activities of soluble protein, soluble sugar, catalase（CAT）,peroxidase（POD）,ascorbate catalase（APX）,superoxide dismutase（SOD） and total flavonoid content increased first and then decreased with the increase of salt concentration, and reached the maximum value at 200 mmol·L^-1 salt concentration.When treated at 100 mmol·L^-1 salt concentration for 1 to 5 days, CBD content was slightly higher than that of CK,but the difference was not significant（P＞0.05）,while under 200-400 mmol·L^-1 treatment, CBD content was continuously decreased with the increase of salt concentration and the extension of treatment time, but there was a significant difference compared with CK（P＜0.05）.These results indicated that C.sativa had a certain tolerance to salt stress.Under salt stress below 200 mmol·L^-1,C.sativa could maintain basic physiological activities, while salt concentration above 200 mmol·L^-1,C.sativa physiology was inhibited and positively correlated with salt concentration.