Abstract: Agrocybe cylindrace were cultivated with Miscanthus floridulus and cottonseed shells as the main raw materials, and design of cultivation formulations according to different addition ratios, the effects of five clinker cultivation formulations on mycelium growth, biological efficiency, agronomic traits and five extracellular enzyme activities of A.cylindracea were studied, in order to provide reference for improving the comprehensive utilization value of mushroom resources such as Miscanthus floridulus and promoting the sustainable development of China′s mushroom industry.The results showed that formulation P3 had the best performance on mycelial growth, agronomic characteristics, first crop yield and biological efficiency of A.cylindracea,these traits were superior to other formulations, with neat and dense mycelial growth, the shortest germination time, the best morphological characteristics of fruiting entities, the first tide mushroom yield of（317.187±1.980） g per bag, and the biological efficiency of 63.44%.The activity changed of extracellular enzymes such as CMCase, xylanase, amylase, laccase and proteinase in the whole growth period of A.cylindracea under different cultivation formulas had obvious stages, and the trend of activity of the same enzyme in different cultivar formulations was generally consistent.The correlation analysis between mycelial growth rate and five extracellular enzymes showed that CMCase, xylanase and amylase were positively correlated with mycelial growth rate, among which xylanase and amylase were highly significantly positively correlated, indicating that the cultivation formula with fast mycelial growth rate could promote the secretion of mycelial extracellular enzymes, and in order to guarantee the supply of nutrients, the mycelium secreted more CMCase, xylanase and amylase to degrade the cultivated material.Comprehensive analysis, the P3 formulation was most suitable for the growth of tea tree mushrooms.