Abstract: With 57 Phalaenopsis germplasm resources collected as research objects, 23 biological traits were investigated and analyzed to clarify the main factors affecting the comprehensive evaluation of the biological traits of Phalaenopsis germplasm resources, and the hierarchical analysis method was combined with the K-Means clustering analysis to establish a comprehensive evaluation system of the biological traits of Phalaenopsis germplasm resources, so as to be able to evaluate the existing germplasm comprehensively and classify existing germplasm resources for reference in the utilization, selection and breeding of new varieties and cultivation promotion of Phalaenopsis.This system was intended to evaluate the existing germplasm in a more comprehensive way, to classify the existing germplasm resources, and to provide reference for the utilization of Phalaenopsis germplasm resources, the selection and breeding of new varieties, and the promotion of cultivation.The results showed that floral morphology（C1） was the main factor in the comprehensive trait evaluation of Phalaenopsis,flower color accounted for the largest proportion, pedicel morphology（C2） was the second largest, and plant morphology（C3） was the least weighted; the highest score in the comprehensive evaluation was ‘Guangmangsishe’（score 4.270 6）;the highest scores in the evaluation of floral morphology were ‘Fortune Cat’ and ‘Guangmangsishe’（score 2.501）,the highest scoring plant form was ‘Longshufengye’（score 0.747 2）,and the highest scoring pedicel form was ‘1713’（score 1.155 4）;57 germplasm were categorized into 4 grades based on the evaluation scores, including 9 excellent grades, accounting for 15.79%;18 good grades, accounting for 31.58%;20 medium grades, accounting for 35.09%;and 10 passing grades, accounting for 17.54%.