Abstract: Taking the young leaves,stems,and hypocotyls of Astragalus membranaceus as the test materials,different concentrations and combinations of 6-BA,NAA,IAA,IBA,and TDZ were added to MS medium to study the establishment of callus and adventitious bud induction differentiation and regeneration systems of Astragalus membranaceus.The aim was to construct a complete system for tissue culture and plant regeneration of Astragalus membranaceus,in order to provide reference for the protection and utilization of Astragalus membranaceus resources,and laid a technical foundation for the further rapid propagation of seedlings and genetic improvement of Astragalus membranaceus.The results showed that young stems were the best explants for callus,and the best medium for callus induction was MS+6-BA 1.0 mg·L^-1+NAA 1.5 mg·L^-1+TDZ 0.2 mg·L^-1,and the induction rate was 90.00%.The optimal medium for inducing adventitious buds from callus was MS+6-BA1.0 mg·L^-1+NAA 0.3 mg·L^-1,and the induction rate was 76.60%.The optimal medium for inducing adventive buds from young stems was MS+6-BA 1.5 mg·L^-1+IAA 0.2 mg·L^-1,and the induction rate was 90.30%.The optimal medium for rooting culture was 1/2MS+IBA 0.5 mg·L^-1+IAA 1.0 mg·L^-1,and the rooting rate was 39.30%.