‘红颜’草莓无糖组培生根技术研究及移栽基质筛选

Research on Rooting Technology of ‘Benihoppe’ Strawberry Sugar-free Tissue Culture and Screening of Transplanting Substrate

摘要: 以‘红颜’草莓为试材，采用无糖组织培养方法，研究了‘红颜’草莓无糖组培生根技术，以期为草莓生产实践提供参考依据。结果表明：无糖组培最佳生根培养基为1/2MS+0.4 mg·L^-1IBA;传统组培最佳生根培养基为1/2MS+0.5 mg·L^-1IBA;培养条件以纯蛭石为基质，光照时长为12 h, CO₂通气时间在3 d时，草莓无糖组培幼苗生长最为健壮；在基质为草炭土∶蛭石∶珍珠岩=3∶1∶1配比下，草莓无糖组培苗移栽成活率达100%。

Abstract: Taking ’Benihoppe’ strawberry as the test material,the rooting technology of ’Benihoppe’strawberry sugar-free tissue culture was studied by using the method of sugar-free tissue culture,in order to provide reference for strawberry production practice.The results showed that the best rooting medium for sugar-free tissue culture was 1/2MS+0.4 mg·L^-1 IBA,the best rooting medium for traditional tissue culture was 1/2MS+0.5 mg·L^-1 IBA,the strawberry sugar-free tissue culture seedlings grew most robustly when the substrate was pure vermiculite as the substrate,the light duration was 12 hours,and the CO₂ ventilation time was 3 days,and the survival rate of strawberry sugar-free tissue culture seedlings was 100% under the ratio of peat-soil:vermiculite:perlite=3:1:1.