Abstract: The ‘Muscat Hamburg’ grape was the main grape variety in Bohai bay area and used as the research material of this experiment,and the difference in fruit quality and phenolic substances of ‘Muscat Hamburg’ grape in Tianjin,Hebei and Shandong areas were determined,using conventional physical and chemical index determination methods and colorimetric methods,so as to provide theoretical basis for the efficient planting of ‘Muscat Hamburg’ grape.The results showed that the contents of soluble solids（18.80%） and total sugar(16.92 g ·（100 g）^-1) were higher in Tianjin,the contents of titratable acid(0.43 g ·（100 g）^-1) and vitamin C(2.69 mg·（100 g）^-1) were higher in Hebei than in other two places,and the sugar-acid ratio was 36.15.The overall quality of ‘Muscat Hamburg’ grape in Shandong was poor.The content of phenolic substances in different parts of rose scented grapes was grape seed＞grape peel＞grape pulp.The content of total proanthocyanidins and total polyphenols in grape seeds in Tianjin was higher than that in Hebei and Shandong.The content of total flavonoids in grape seeds in Shandong was higher than that in the other two places.In conclusion,compared with Hebei and Shandong,the quality of‘Muscat Hamburg’grape in Tianjin was better,the phenolic substances were relatively abundant,and the fruit quality in shandong was poor.