Flowering Induction of F₁ Generation of Cymbidium hybridium ‘Dafeng’×C.serratum var.goeringii ‘Taijishengmei’ in vitro

The F₁ generation plantlets of Cymbidium hybridium ‘Dafeng’ × C.serratum var.goeringii ‘Taijishengmei’ were used as test material pretreated with PP₃₃₃ or CCC,and subsequently added with different concentrations of 6-BA to determine plant morphology and physiological indicators, a test-tube flowering induction system was established and the childhood period of orchid plants was shortened, in order to provide reference for breeding of orchid.The results demonstrated that pretreatment with different concentrations of PP₃₃₃ or CCC inhibited the increasing of plant height, root number and fresh weight, but promoted leaf division.The test tube seedlings were pre-cultured in 1/2MS+PP₃₃₃ 1.0 mg·L^-1+banana puree 120 g·L^-1+sucrose 30 g·L^-1+agar 8 g·L^-1+activated carbon 0.5 g·L^-1 for 30 days.Then, the culture medium of 1/2MS+NAA 0.2 mg·L^-1+banana pulp 120 g·L^-1+sucrose 30 g·L^-1+agar 8 g·L^-1+activated carbon 0.5 g·L^-1 could induce the flower bud flowering, the flower bud induction rate was 6.67%,but the flower bud did not open successfully in the late stage.The contents of soluble sugar and soluble protein were higher than CK under PP₃₃₃ or CCC pretreatment, which promoted the increase of C/N value and the decrease of total nitrogen content.