Antimicrobial Activity of Endophytic Fungi BB01 Derived From Blueberry

‘Pink blue’ blueberry collected from Majiang cultivation base in Guizhou was used as the test material.The isolation and purification experiment, biological identification, confrontation test,mycelial growth test and resazurin microtitre plate antibacterial assay were performed to study the antibacterial activity of blueberry endophytic fungus BB01,in order to provide a research foundation for the development of green biological preservatives.The results showed that 41 strains endophytic fungi were isolated from the healthy tissue（stem, leaf, and fruit）,among which BB01,BL22,BF27,BB01 and BF41 could inhibit the growth of all 4 pathogens.Endophytic fungus BB01 was undergone the process of liquid fermentation to obtain broth ethyl acetate extract（BE）,it was found that CE exhibited the best inhibition against Penicillium,with minimum inhibitory concentrations（MIC） values of 64 μg·mL^-1,and BE exhibited the best inhibition against Cladosporium,Glomerella acutata and Botrytis cinerea,with MIC values of 16 μg·mL^-1,256 μg·mL^-1 and 128 μg·mL^-1,respectively.Moreover BE also exhibited 98.067 302%±3.813 437%,92.187 892%±0.828 473%,94.251 883%±0.368 539%,96.909 029%±5.769 104% growth inhibiton against 4 pathogens respectively at 500 μg·mL^-1.PCR was used to amplify the genome rDNA-ITS sequence for phylogenetic tree.The results showed that BB01 was Cladosporium sp.