Establishment and Preliminary Application of PCR Detection Methods for Pantoea pleuroti

Pantoea pleuroti,blight disease pathogen of Pleurotus eryngii was used as the test material, PCR primers were designed, synthesized and screened after analyzing the sequencing results of P.pleuroti genome, and the detection effect was studied, so as to establishefficient PCR detection system for P.pleuroti and provide molecular basis for scientific control of blight disease of P.eryngii.The results showed that the specificity of K3143 f/R and K37 f/R primers were strong, only when P.pleuroti genomic DNA was used as template, the PCR products showed a specific band of 660 bp and 666 bp, respectively, while the genomic DNA of 15 strains of Pantoea,three pathogens strains of common edible fungi disease and distilled water（negative control） were used as template, the PCR products showed no band.The detection systems based on these two primer pairs were of high sensitivity and were not interfered by the tissue fluid of P.eryngii,which could detect the lowest 3.6 pg·μ L^-1 P.pleuroti genomic DNA.Furthermore, at least 100 cfu P.pleuroti could be detected when P.pleuroti had been inoculated onto the fruiting bodies of P.eryngii for 48 hours.In addition, primer K3143 F/R was of higher efficiency than that of K37 F/R.