Cloning, Sequencing and Expression Analysis of Rosa multiflora Rm4CL Gene

Taking the wild rose（Rosa multiflora） as the test material, the genome of the 4CL1α1 gene and the full-length CDS clone were studied by PCR and RT-PCR,in order to provide reference for the anabolic pathway of rose flower color.The results showed that the expression of the 4CL gene family in different tissues of rose and after hormone induction was analyzed by semi-quantitative qRT-PCR. The 4CL1α1 gene was cloned, with gDNA length 4 261 bp and CDS length 1 632 bp, encoding 543 amino acids. Through bioinformatics analysis, it was preliminarily determined to be 4CL or 4CL-like. Through the real-time quantification of fluorescence of 6 genes, it was found that the expression of the 4CL gene family was relatively high in safflower and young thorns. It was preliminarily determined that the Rm4CL gene family in roses plays an important role in anthocyanin synthesis and cell wall thickening.