Tissue Culture Technology of Malus ‘Yunxiangrong’ Stem

The effects of different disinfectant, time of disinfection, concentration of auxin and cytokinin on tissue culture of Malus ‘Yunxiangrong’ were studied by tissue culture method, in order to establish rapid propagation system of Malus ‘Yunxiangrong’.The results showed that the best disinfection method of primary culture was 75% alcohol disinfection for 30 seconds, and treatment with 0.1% mercuric chloride 8 minutes, this disinfection method could obviously reduce the contamination rate of explants, the damage caused by external implants was small, the survival rate was as high as 83.3%.Best axillary bud induction medium MS+6-BA 1.0 mg·L^-1+NAA 0.1 mg·L^-1,bud growth robust and induction rate up to 90%.The one-step rooting method was better with the medium 1/2 MS+IBA 1.0 mg·L^-1,with an average root length of 5.26 cm,and a rooting rate of 73.3%.In comparison,the two-step rooting method could effectively promote the rooting of the begonia,and the rooting rate could reach 91.67%.