Contact Toxicity to Myzus persicae （Sulzer） of Ethanol Crude Extract of Aquilegia viridiflora Pall

Columbine（Aquilegia viridiflora） was taken as the research material.Peach aphid（Myzus persicae） was selected as the trial target.The insect immersion method was applied to get the estimated values of mortality and adjusted mortality of peach aphid treated by ethanol crude extract of whole columbine of different growing periods and different concentration.R language was used to analyze significant differences and to calculate toxicity regression equations.So the laboratory contact toxicity of ethanol crude extract of columbine was studied, which was supposed to provide theoretical basis for columbine as a new source of botanical insecticide.The results showed that, the ethanol crude extract of columbine in all growing periods had contact toxicity of different levels to peach aphid.The longer the treatment time was, the more obvious the effect was.Under the same treatment concentration and time, the blooming ethanol extract had the highest insecticidal activity, which was significantly different from that at production and seedling.The toxicity regression equation of blooming crude extract against peach aphid was y=1.57+2.22x,R~2=0.87 and LC₅₀=35.1 mg·mL^-1 after 48 h, and y=1.5+2.53x,R~2=0.9 and LC₅₀=24.2 mg·mL^-1 after 72 h.