Seedling Regeneration From Isolated Microspore-derived Embryo in Cabbage

Twenty different genotypes of cabbage were used as test materials for isolated microspore culture to study the influencing factors of microspore embryogenesis and plant regeneration, and to identify the ploidy and seed setting of regenerated plants, in order to establish an efficient isolated microspore culture system and provide reference for rapid innovation of germplasm materials in the later stage.The results showed that the genotype had a significant impact on the embryoid induction.The highest embryo yield was 42.33 embryo·bud^-1 in inbred line ‘D3’.Additionally, the embryo yield of this variety was 17 folds that the lowest embryo yield of variety ‘D9’.The yield of microspore embryo was the highest when the ratio of petal length and anther length ranged from 0.51 to 0.75.The addition of 0.10-1.00 mg·L^-1 TDZ significantly increased the yields of all type embryo, and the obtained cotyledon embryos had strong seedling capacity.Flow cytometry detection of 303 regenerated plant revealed that 49.18% were haploid, 43.89% were doubled haploid, and chimera only accounted for the remaining 6.93%.Double haploid and chimeric plants could set pods normally after pollination at the bud stage, while the haploid plants could not produce pods.