Effects of Colchicine Mutagenesis on Growth and Active Ingredients in Hyphae of Phellinus igniarius

Phellinus igniarius hyphae treated with 1% cellulase and 0.5% snail enzyme for different time were used as materials.Growth rate and changes of active ingredients such as polysaccharides, flavonoids and triterpenes of Phellinus igniarius mycelial regeneration strains inducedby 0.1% colchicine were analyzed, in order to obtain strains with fast growth and high content of active ingredients and lay the foundation for the selection and breeding of Phellinus igniarius strains, innovation and application of germplasm resources.The results showed that the growth rate and the content of active ingredients such as polysaccharides, flavonoids and triterpenes were basically increased after colchicine treatment.Among them, compared with the control, the regenerated strain 20-1 obtained by mutagenizing the hyphae of Phellinus igniarius for 20 minutes had the fastest growth rate, which increased by 366.67%.The regenerated strains with the highest content of triterpenes and polysaccharides were 10-5,which increased by 1 069.73% and 281.85%,respectively.The regenerated strain with the highest polyphenol content was 5-1,which increased by 530.56%.The regenerated strain with the highest flavonoid content was 30-1,which increased by 3 771.43%.