Cloning and Expression Analysis of Flavanone 3-hydroxylase SlF3H-like Gene From Purple Tomato

The cherry purple tomato ‘Yanzi No.1’ was used as test material.RT-PCR,bioinformatics and qRT-PCR expression analysis were used to study the biological information of flavanone 3-hydroxylase gene SlF3 H-like,in order to characterize the sequence characteristics and expression pattern of the gene.The results showed that the open reading frame（ORF） of SlF3H-like gene was 1 089 bp, encoding 362 amino acids.The random coil was found to occupy the majority of structural elements of SlF3 H-like protein, followed by α-helix and extended chain.The F3 H amino acid sequence of ‘Yanzi No.1’ was highly conserved from other species.SlF3 H-like protein contained typical DIOX-N superfamily and 2 OG-FeⅡ＿Oxy domains.Phylogenetic analysis showed that ‘Yanzi No.1’ was closely related to ‘Pannali’ tomato, followed by potato.The anthocyanin content in leaves and pericarp of ‘Yanzi No.1’ was measured during its development.It was found that the anthocyanin content in pericarp gradually accumulated with the increase of fruit diameter, while the anthocyanin content in leaves increased first and then decreased sharply with the increase of leaf diameter.qRT-PCR was used to analyze the expression of SlF3 H-like gene in the pericarp and leaves of ‘Yanzi No.1’.Results showed that the change trend of this gene expression was similar to that of anthocyanin content, indicating that SlF3 H-like gene played an important role in anthocyanin accumulation of purple tomato.