Study on Rapid Technique of Tissue Culture of Ranunculus asiaticus

The sterile seedlings of Ranunculus asiaticus were cultured on MS medium supplemented with 6-BA and NAA respectively to induce and proliferate in the primary generation of Ranunculus asiaticus,the experiment of Ranunculus asiaticus seedlings transplanting was carried out by subculture of rooting and different proportion of peat,vermiculite,perlite and river sand.The results showed that the best medium for bud induction was MS+1.5 mg·L^-1 6-BA+0.2 mg·L^-1 NAA,the induction rate was 100%,the medium for callus induction was MS+0.5 mg· L-1 6-BA +0.1 mg·L-1 NAA,the multiplication coefficient was 5.0.The subculture medium for rooting was1/2 MS+0.2 mg·L-1 NAA,the rooting rate was 89.9%,the best medium for transplanting was peat+river sand+vermiculite=6∶2∶2,survival rate was 100%.