Establishment of in vitro Regeneration System of Lilium regale Wilson From Flower Organs

Lilium regale Wilson was used as the test material, the filaments, styles and ovules were used as explants for tissue culture.Adventitious bud or callus induction, callus subculture, bulb expansion and rooting, and seedling training and transplanting of test tube plantlets were studied in order to provide reference for the establishment on the tissue culture and rapid propagation technology of flower organs of Lilium regale Wilson.The results showed that the induction ability of explants of three flower organs was different, the order of induction ability from easy to difficult was filament＞style＞ovary.Filaments were the most prone to browning, while ovaries were the least prone to browning.The optimal medium of different explants was different.The most suitable medium for filament explants was MS+BA 1.0 mg·L^-1+NAA 0.5 mg·L^-1 with the highest induction rate reaching 91%,the best medium for style induction was MS+2,4-D 1.0 mg·L^-1+KT 0.1 mg·L^-1,showing 63% induction rate.Two kinds of callus were produced in the process of flower organ induction, and the best explants for ovary induction was MS+2,4-D 2.0 mg·L^-1+KT 0.05 mg·L^-1,with 52% induction rate.BA and NAA were mainly induced to produce green, dense and hard callus, which were identified as non-embryonic callus by paraffin section; 2,4-D and KT were mainly induced to produce yellow, loose and granular callus, which were identified as embryonic by paraffin section.The embryo callus was subcultured, the most suitable subculture medium was MS+PIC 1.0 mg·L^-1+NAA 0.2 mg·L^-1;while the most suitable medium for small scale stem enlargement and rooting was MS+90 g·L^-1 sucrose; the larger the bulb（＞10 mm）,the higher the transplant survival rate（90%）,the better the plant growth.