Abstract: In this study, a vegetable protein beverage was prepared with Cerasus humilis kernel as materials, and the nutritional properties were investigated. On the basis of single factor tests, orthogonal test was used to determine the optimal formula of Cerasus humilis kernel vegetable protein drink, and the results were determined as follows: solid-liquid ratio of 1∶20（g/mL）, sucrose addition of 4%, sucrose fatty acid ester addition of 0.06%, and xanthan gum addition of0.10%. The beverage prepared with this formula contained 14 kinds of amino acids with total amount of 0.955 mg/mL,and the total amount of 6 kinds of essential amino acids（threonine, valine, isoleucine, leucine, phenylalanine, lysine）was 0.336 mg/mL, accounting for 35.18% of total amino acids. At the same time, there were 7 fatty acids including linoleic acid, oleic acid, palmitic acid, eicosapentaenoic acid（EPA）, stearic acid, tridecanoic acid and docosadienoic acid, and the contents of oleic acid and linoleic acid were 59.80 mg/100 g and 66.40 mg/100 g, respectively. The beverage product prepared with the above formula presented milky white, strong flavor, good stability with high nutritional value and health function.