Abstract: In the present study, the residual changes of different concentrations of chlorothalonil in sugarcane were determined using yellow skin fruit sugarcane as the experimental material, aiming to securely and efficaciously use chlorothalonil and investigate the residual changes during storage. The detection method referred to NY/T 761—2008.The 100, 200, 300, 400, 500 times levels solution was prepared using 75% chlorothalonil wettable powder, which was sprayed on the stored fruit sugarcane, and the juice of fruit sugarcane was extracted every 3 days to determine the chlorothalonil content in chewing cane. The results showed that, the average recovery rates of chlorothalonil were 100% ～103% and RSD values were 0.24% ～1.71%. The maximum and minimum residues of chlorothonil in cane juice were 1.24 mg/kg and 0.07 mg/kg, respectively, during 39 days storage. The method for chlorothalonil determination by gas chromatography showed good operability, superior repeatability and high sensitivity, which was suitable for the detection of chlorothalonil in this experiment.