响应面优化蕲艾总黄酮提取工艺及其抗氧化活性研究

Extraction Process of Total Flavonoids from Artemisia argyi Optimized by Response Surface Methodology and the Antioxidant Activity Research

摘要: 以蕲艾为试验原料,采用微波辅助法提取总黄酮,并应用响应面分析法优化蕲艾总黄酮的提取工艺。结果表明:在微波功率400 W、微波时间5 min、料液比1∶80（g/mL）的条件下,蕲艾中总黄酮得率可高达14.22%。对在该最优工艺条件下提取的蕲艾总黄酮进行抗氧化活性研究,试验结果表明:蕲艾中总黄酮的质量浓度与其对DPPH自由基的清除能力和体外总还原能力均呈线性关系,当质量浓度为1.0 g·mL^-1时,黄酮的DPPH自由基清除能力和体外总还原能力达到最强。

Abstract: In this study, the Artemisia argyi was used as test material, and the total flavonoids were extracted under microwave assistance, then the extraction process was optimized by response surface analysis. Results showed that the optimal extraction conditions were determined as follows: microwave power of 400 W, microwave time of 5 min, material-liquid ratio of 1∶80（g/mL）, under these conditions, the extraction yield of total flavonoids was 14.22%. The antioxidant analysis results showed that the mass concentrations of total flavonoids in Artemisia argyi presented a linear relation with scavenging ability of DPPH free radical and total reduction ability in vitro, and the total flavonoids with mass concentration of 1.0 g/mL displayed the strongest DPPH free radical scavenging ability and total reducing power in vitro.