Abstract: In order to provide evidence for tomato quality grading, five tomato varieties were used as experimental materials by analyzing the quality indexes including soluble solids, soluble sugar, titratable acid and etc., to determine the quality difference between tomato varieties during five harvest time. The results suggested that there were significant differences in the quality of five tomato varieties. The average contents of soluble solid, titratable acid,soluble sugar and VC of ‘Saopolo’ were 8.92%, 7.35%, 0.66% and 4.57 mg/g, respectively, which were significantly higher than the other varieties except ‘Jua’. The average contents of soluble solid and soluble sugar of ‘Harvest560’ were the lowest, only 3.64% and 2.04% respectively. There were certain differences on tomato quality during multiple harvest time, including soluble solid, solid acid ratio, VC and a*value, but had no significant differences on the soluble sugar content, titratable acid content, sugar acid ratio, L*value, hardness and sensory quality. Therefore,soluble sugar content, titratable acid content, sugar and acid ratio, L*value, hardness and sensory quality should be used as grading indexes to reduce the influence of harvest periods.