豆渣水溶性膳食纤维的复合酶法提取及其应用于可食性膜研究

Extraction of Water-Soluble Dietary Fiber from Soybean Residue by Compound Enzymatic Method and Its Application in Edible Film

摘要: 以豆渣为原料，通过膨化辅助复合酶解法提取豆渣可溶性膳食纤维（SDF），然后将其制成可食性膜，并利用正交试验对豆渣SDF的提取条件和成膜条件进行优化。结果表明，豆渣SDF最佳提取条件为：豆渣与蒸馏水之比1∶30(g∶mL)，纤维素酶添加量30 g/L（以蒸馏水添加量计），酶解时间2 h，蛋白酶酶解体系pH值为9.0；最佳成膜条件为：豆渣SDF 1.0 g,CMC-Na添加量1.0 g，海藻酸钠添加量1.5 g，甘油添加量4.5 mL。该条件下制得的豆渣可食性膜不但光滑，而且性质稳定，水溶解速度为0.098 mg/s，水滴漏时间为12 060 s。

Abstract: Soluble dietary fiber（SDF） was extracted from soybean residue by expanding-assisted complex enzyme hydrolysis, and then made into edible film. The extraction and film-forming conditions of bean residue SDF were optimized by orthogonal tests. The results showed that the optimum extraction conditions were as followed: ratio of bean dregs to distilled water 1∶30（g∶mL）, cellulase addition 30 g/L（calculated by distilled water volume）, extraction time 2 h, and protease enzymolysis p H 9. The best film forming conditions were: soybean dregs SDF 1.0 g, CMC-Na1.0 g, sodium alginate 1.5 g and glycerol 4.5 mL. Under the above conditions, the prepared soybean residue edible film had smooth and stable properties, with water dissolution rate of 0.098 mg/s and water drip time of 12 060 s.