Abstract: The stems of naturally growing Anredera cordifolia were used as raw materials, and total flavonoids was extracted by microwave method with different concentrations of ethanol as extractant. Based on single-factor experiments, the effects of different parts, material-liquid ratio, ethanol concentration, microwave power and time on the extraction rate of total flavonoids from Anredera cordifolia were investigated, and the order of influencing factors and optimal extraction technique were determined. The result showed that the content of total flavonoids in stem of Anredera cordifolia was higher than that in other parts, and the stem was not easily oxidized. The best extraction technique of total flavonoids from Anredera cordifolia stem was as follows: microwave time 70 s, microwave power 320 W, ethanol concentration 70%, and material-liquid ratio 1 ∶80（g/mL）. Under these conditions, the extraction yield was 7.42%. The main factors affecting the extraction rate were microwave power and ethanol concentration, which should be controlled during production and processing.