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外源阿魏酸抑制鲜切苹果褐变机制研究

Mechanism of Browning Inhibition of Exogenous Ferulic Acid on Fresh-Cut Apples

  • 摘要: 苹果在鲜切过程中易因遭受机械损伤而引发褐变,严重影响其感官品质、风味和商品价值。采用不同浓度外源阿魏酸溶液对鲜切‘红富士’苹果进行浸泡处理,以研究其抑制鲜切苹果褐变的机制。结果表明:1、5、8、10 g/L的阿魏酸浸泡处理均能有效抑制鲜切苹果褐变,10 g/L阿魏酸浸泡处理10 min对鲜切苹果褐变的抑制效果最优;10 g/L阿魏酸处理显著提高了鲜切苹果超氧化物歧化酶(SOD)活性及1,1-二苯基-2-三硝基苯肼(DPPH)自由基、2,2-联氨-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)阳离子自由基的清除率,抑制了贮藏后期多酚氧化酶(PPO)活性,降低了表儿茶素等单一酚的含量,提高了对香豆酸的含量;添加5 mg/mL表儿茶素会促进苹果浆液褐变,而添加5、10、20、40 mg/mL对香豆酸均能抑制苹果浆液褐变。综上所述,外源阿魏酸处理可通过提高SOD活性、抗氧化能力、内源褐变抑制物含量以及降低褐变底物含量来减轻鲜切苹果褐变。

     

    Abstract: Apples can be mechanically injured during fresh-cutting process, which can cause browning and seriously affect the sensory quality, flavor, and commercial value. In this study, the fresh-cut ‘Red Fuji’ apples were soaked under different concentrations of exogenous ferulic acid solutions, in order to study the mechanism of browning inhibition. The results showed that soaking with 1, 5, 8, and 10 g/L of ferulic acid could inhibit the browning of fresh-cut apples, and soaking with 10 g/L of ferulic acid for 10 min presented the best inhibitory effects. The treatment with10 g/L ferulic acid significantly increased the superoxide dismutase(SOD) activity and the clearance rates of 1,1-diphenyl-2-picrylhydrazyl(DPPH) free radical and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)cation radicals in fresh-cut apples. It also inhibited the polyphenol oxidase(PPO) activity at the later stage of storage,reduced the single phenol contents such as epicatechin, and increased the coumaric acid content. Epicatechin addition with 5 mg/mL could promote apple juice browning, while coumaric acid addition with 5, 10, 20, 40 mg/mL could all demonstrated inhibitory effects. In summary, exogenous ferulic acid treatments could alleviate the browning of fresh-cut apples via increasing SOD activity, antioxidant capacities, the contents of endogenous browning inhibitors,and reducing the contents of browning substrates.

     

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