Abstract: The strain A4 was identified depended on the morphology, molecular biology and physiological and biochemical characteristics, and the control effects of A4 on postharvest diseases of cherry tomato were investigated.Results showed that strain A4 was identified as Bacillus velezensis, and exhibited inhibitory effects against Botrytis cinerea and Penicillium expansum. The plate confrontation test showed that the inhibition rates of Bacillus velezensis A4 on the growth of above two pathogens were 66.56% and 59.19%, respectively, and the prevalence of cherry tomato was decreased to 14.67% and 4.00% respectively under the pre inoculation concentration of 1.0×10~9CFU/mL. Scanning electron microscope results showed that biocontrol strain A4 inhibited the germination and growth of pathogens, reduced pathogenicity and diseases incidence. This research could provide a reference for the identification of biocontrol bacteria Bacillus velezensis, and a new strategy for the control on postharvest diseases of cherry tomato.