Abstract:
In this paper,the autumn red T.sinensis buds were taken as test materials,and the anthocyanins and chlorophylls contents during refrigeration were measured,the anthocyanin synthesis genes were identified using transcriptome data,and the relative expressions of genes related to anthocyanin synthesis were analyzed using real-time fluorescence quantitative PCR,aiming to study the change mechanisms of anthocyanins in red T.sinensis buds during refrigeration.The results showed that 9 anthocyanin-related genes(TsPAL,TsC4H,TsCHS,TsCHI,TsF3H,TsF35’H’,TsDFR,TsANS and Ts3GT) were identified by transcriptome.In autumn,the contents of anthocyanins and chlorophylls in leaves were higher than those in petioles.With the extension of cold storage period,the contents of anthocyanins in leaves were degraded,and the contents of chlorophylls were increased at 3 days.Except for TsPAL and TsC4H genes,the relative expression levels of other anthocyanins genes in red T.sinensis buds showed an overall downward trend during cold storage.Correlation analysis showed that the relative expression levels of TsPAL,TsC4H and TsCHS genes in petioles were negatively correlated with anthocyanins contents,while the relative expression levels of the remaining genes were positively correlated with anthocyanins contents.