Abstract: In the present study, the ultrasonic-enzyme assisted extraction（UEAE） process of chlorogenic acid from honeysuckle was optimized by single factor experiment and response surface methodology coupled genetic algorithm using honeysuckle as the raw material, and the effects of chlorogenic acid extract of honeysuckle on ABTS+radical and hydroxyl radical scavenging abilities were investigated. Results showed that the optimum extraction parameters to achieve the highest chlorogenic acid extraction yields 12.85% ±0.37% from honeysuckle by UEAE were obtained under ultrasonic power of 157 W, extraction time of 30 min, extraction temperature of 34 ℃, pectinase addition of 0.20% and ethanol volume fraction of 61%. The in vitro antioxidant activity results showed that chlorogenic acid extract of honeysuckle had strong antioxidant activities, and IC₅₀ values of ABTS+radical and hydroxyl radical scavenging rates were（6.18±0.07） mg/mL and（4.75±0.06） mg/mL, respectively. The results provided a feasible method for the extraction of chlorogenic acid from honeysuckle.